Chemistry Department Central Facility (CenFac)

Instrument information

Brand: Agilent Technologies

Model: G6530A

Brief description: LC-MS is a powerful technique used for many applications which has very high sensitivity and selectivity. Generally its application is oriented towards the general detection and potential identification of chemicals in the presence of other chemicals (in a complex mixture). Preparative LC-MS system can be used for fast and mass directed purification of natural-products extracts and new molecular entities important to food, pharmaceutical, agrochemical and other industries. The Agilent 6530 Accurate-Mass Q-TOF LC/MS systems provide superior data quality and advanced analytical capabilities for profiling, identifying, characterizing, and quantifying low molecular-weight compounds and biomolecules with confidence. It gives wide mass range, m/z20 – 20,000 and mass accuracy is 1-2 ppm MS, and 2-4 ppm MS/MS.

Techical specification

1. Operating Software: Mass Hunter

2. Detector: Mass Spectrometer

3. Column: Hardware Kit ZORBAX Eclipse XDB-C18

4. HT Cart Hardware Kit Rapid Resolution: HT 2.1X50 mm 1.8 Micron 600 Bar

5. Max volume for loading sample: Depending auto sampler needle assemble (~ 40 Microliter)

6. Max and Min concentration of sample: Concentration < 20 ppm (~5 ppm preferred)

Location

Room C013, Block A Chemistry Department

Person in- charge

Supervisor	Senior Science Officer	Senior Assistant Science Officer

Name:Associate Prof.Dr. Tay Kheng Soo Designation: Associate Professor Telephone: +603-79677022 ext 2145 Fax :None Email: khengsoo@um.edu.my	Name: Miss Norzalida Zakaria Designation: Senior Science Officer Telephone: +60379674245 Fax :None Email: norzalida@um.edu.my	Name: Miss Zailawati Mohamad Zakaria Designation: Assistant Science Officer Telephone: +603-79677022 ext: 2120 Fax :None Email: zailawati@um.edu.my

Supervisor

Senior Science Officer

Senior Assistant Science Officer

Name:Associate Prof.Dr. Tay Kheng Soo
Designation: Associate Professor
Telephone: +603-79677022 ext 2145
Fax :None
Email: khengsoo@um.edu.my

Name: Miss Norzalida Zakaria
Designation: Senior Science Officer
Telephone: +60379674245
Fax :None
Email: norzalida@um.edu.my

Name: Miss Zailawati Mohamad Zakaria
Designation: Assistant Science Officer
Telephone: +603-79677022 ext: 2120
Fax :None
Email: zailawati@um.edu.my

Application Form

Sample requirement

1. Samples should be pure components after chromatography or semi-pure fractions after chromatography. Please ensure that samples introduced have been subjected to at least some degree of purification or pre-treatment.

2. Sample preparation according to the following guidelines.

Sample concentration: < 20 ppm (~ 5 ppm preferred)

1 ppm = 1mg of solute

1L solution

3. Injection volume: < 5µL

4. Filter both your samples and solvents with a 0.2µm nylon membrane

5. Flush the column with 100% MeOH or 100% Acetonitrile for at least 15 minutes after completion of your run.

Service Charge

For futher information regarding service charge, please click HERE.

Liquid Chromatography High Resolution QTOF System